ICISworkbook: Generating the F1 crosses

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The succeeding steps will help you learn how to create your F1 list in ICIS Workbook. There are several ways the F1 list can be formed. One may want to specify the particular crosses to be made in advance. Another way is by checking the HB periodically and crosses could be made according to flowering synchronization. Sometimes, users only want to enter their successful crosses. All variations are possible and crosses can be made interactively or by batch using ICIS Workbook.

1. Click List ► Generate Crosses.

Fig. 1. Generating Crosses

Two windows will appear on each side of the screen. On your left, you’ll see the List Explorer window (see Fig. 2) where you can select the germplasm list which will be the source of your parent germplasms. On your right, you’ll see the Generate Crosses window (see Fig. 2) which gives you the control in creating your F1 list.

2. First, you should select the source germplasm list for the parents by double clicking the list name in the treeview at the left. Note that it is possible for your parent germplasms to come from different list sources. In this exercise, double click the "U03WSHB" list that you created in the first part of this training.

Fig. 2. Selecting source germplasm list for the parents

An Open Template dialog box will appear asking for a list template that you may want to use. In this case, you will not be using any template, hence, click [Cancel].

3. The U03WSHB list will be opened and you will be prompted to define the Naming Convention of the new crosses to be made.

a. Enter the information (found in Table 1) in the Naming Convention - DESIGNATION window.

                                                                                                   Table 1. Entries Naming Convention - DESIGNATION

Prefix	IR
Start No.	90599

b. Upon clicking [Apply], the Naming Convention for the ENTRYCODE of the new crosses would be defined next. In this case, enter the entries (Table 2) in the Naming Convention.

                                                                                                    Table 2. Entries for Naming convention - ENTRYCODE

Prefix	P
Start No.	1
Fixed Width	True
Width Size	3

c. When you click [Apply], Naming Convention for the SOURCE of the new crosses will be asked. This time, go to the [Combination] tab. Check the List Name and ENTRYCODE boxes. Then, click [Apply].

Fig. 3. Naming Convention for the Source

4. At this point, you must now specify the female and male parents. First, select the female parents in the active worksheet by selecting the first eight cells in the DESIGNATION column that contains the names of the non-IR lines. Then, click the [Female Parent] button in the Generate Crosses window to pickup the needed information from the active worksheet.

Fig. 4. Selecting female parents

5. Select the remaining eight IR lines in the DESIGNATION column and click the [Male Parent] button in the Generate Crosses window to get the needed information from the active worksheet.

Fig. 5. Selecting Male Parents

When both parents have already been selected, the List Explorer window disappears along with the source worksheet and a new list template is created to contain the resulting new crosses.

Fig. 6. New List template

6. You must now specify the germplasm properties of the new crosses at the lower half of the Generate Crosses window. For this exercise, specify the information (indicated in Table 3) in the Generate Crosses window.

                                                                                 Table 3. Germplasm properties

Name Type	CROSS NAME
Date Named	20040110
Name Location	IRRI-INTERNATIONAL RICE RESEARCH INSTITUTE
Creation Method	SINGLE CROSS
Date Created	20040110
Germ. Location	IRRI-INTERNATIONAL RICE RESEARCH INSTITUTE

Fig. 7. Generate Crosses window

7. You have the option to do a one-by-one (interactive) cross generation or a batch process through the [Generate Cross] button or the [Generate All Crossess] button, respectively. In this exercise, click the [Generate All Crosses] button.

8. However, there is already an existing germplasm in the database with the name IR 90599 which came from your previous setting of the naming convention for the DESIGNATION of the new crosses. So, the Germplasm Name Already Exist window will appear informing you of the situation and giving you different options on how to proceed. For this exercise, select the option [Use the next derivative name] and click [OK to All] to apply the selected option to all the other new germplasms that might also encounter a name conflict.

Fig. 8. Germplasm Name Already Exist window

9. Then, 64 new germplasm names will be registered into the database as a result of the crossing process. You will be notified of the success of the operation through a message box.

Fig. 9. Generate Crosses message box

10. Upon clicking [OK],you will be directed to the Description sheet where you can now specify the information (found in Table 4) to prepare for loading the germplasm list.

                                                                                                  Table 4. Information for loading germplasm list

LIST	U04DSF1
TITLE	Upland F1 Nursery 2004 Dry Season
DATE	20040110
TYPE	F1

11. Lastly, click List ► Load List and do the succeeding procedures as what you've done during the first exercise of this training.